Affinity separations a practical approach

Cover of: Affinity separations |

Published by Oxford University Press in Oxford, New York .

Written in English

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Subjects:

  • Affinity chromatography -- Laboratory manuals.

Edition Notes

Includes bibliographical references and index.

Book details

Statementedited by Paul Matejtschuk.
SeriesThe practical approach series
ContributionsMatejtschuk, Paul.
Classifications
LC ClassificationsQP519.9.A35 A355 1997
The Physical Object
Paginationxix, 253 p. :
Number of Pages253
ID Numbers
Open LibraryOL1011269M
ISBN 10019963551X, 0199635501
LC Control Number96051855

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Affinity Separations: A Practical Approach (Practical Approach Series) [Matejtschuk, Paul] on *FREE* shipping on qualifying offers. Affinity Separations: A Practical Approach (Practical Approach Series). Affinity chromatography uses very specific interactions between the compound of interest and a ligand bound to a chromatographic support to obtain separations.

An early example of affinity separations was the use of an enzyme and its substrate. One particular type of. Affinity separations, and especially affinity chromatography, have become popular tools for the selective purification and isolation of biological compounds, recombinant proteins and biopharmaceuticals.

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Winzor Affinity separations of proteins / Paul Matejtschuk, Peter A. Feldman and Jacky Rott [and others] Affinity separations of nucleic acids / Ram P. Singhal and Chenna R. Chakka Affinity chromatography of oligosaccharides and glycopeptides / Richard D.

Cummings ♥ Book Title: Methods for Affinity-Based Separations of Enzymes and Proteins ♣ Name Author: Munishwar N. Gupta ∞ Launching: Info ISBN Link: ⊗ Detail ISBN code: ⊕ Number Pages: Total sheet ♮ News id: oA6M0m_yIEMC Download File Start Reading ☯ Full Synopsis: "One major concern of biotechnology is either using enzymes or producing.

Design and scale-up of affinity separations. (OCoLC) Material Type: Document, Thesis/dissertation, Internet resource: Document Type: Internet Resource, Computer File: All Authors / Contributors: Frances Hamilton Arnold.

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Download Citation | Affinity Separations | IntroductionGeneral Strategies for Analytical MethodsAnalytical Methods SummaryReferences | Find, read and cite all the research you need on ResearchGate.

The full text of this article hosted at is unavailable due to technical difficulties. This book presents a comprehensive overview focused specifically on the present state, future challenges and opportunities for separation and purification methods and technologies in biorefineries.

Topics covered include: Equilibrium Separations: Distillation. Affinity separations book Depending upon the procedure used for isolating the ligand-ligate complex, the process can be termed affinity ultrafiltration, affinity partitioning or affinity precipitation.

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AFFINITY CHROMATOGRAPHY USING PURIFICATION TAGS 7 Rationally Designed Ligands for use in Affinity Chromatography: an Artificial Protein L Ana Cecí­lia A. Roque and Christopher Lowe 8 Phage display of Peptides in Ligand Selection for use in Affinity Chromatography Joanne L Casey, Andrew M.

Coley and Michael Foley 9 Preparation, Analysis and Price: $ The trilogy: Individually download our three volumes of Affinity Chromatography handbooks. Protein purification: types. Affinity Chromatography - Vol.

1: Antibodies. Affinity Chromatography - Vol. 2: Tagged Proteins. Affinity Chromatography - Vol. 3: Specific Groups of Biomolecules. Hydrophobic Interaction Chromatography.

Book Description. This essential handbook guides investigators in the theory, applications, and practical use of affinity chromatography in a variety of fields including biotechnology, biochemistry, molecular biology, analytical chemistry, proteomics, pharmaceutical science, environmental analysis, and.

Expanded and updated from the first edition, Affinity Chromatography: Methods and Protocols, Second Edition, provides the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era.

This second edition expands on the first edition by introducing more state. Biotechnology (N Y). Feb;9(2) Metal-affinity separations: a new dimension in protein processing. Arnold FH(1). Author information: (1)Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena Rapid growth in the preparative and high-resolution analytical applications of metal-affinity chromatography demonstrate the appeal of metal Cited by: Affinity Photo’s HDR algorithm creates incredibly natural-looking images enabling you to unlock the full dynamic range of the scene.

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Methods and Tools in Biosciences and Medicine. Share your thoughts Complete your review. Tell readers what you thought by rating and reviewing this book. Rate it * You Rated it *Brand: Birkhäuser Basel. Affinity in Book Titles. Affinity, That Elusive Dream: A Genealogy of the Chemical Revolution.

Methods for Affinity-Based Separations of Enzymes and Proteins. Handbook of Affinity ChromatographyWhat Kinship Is-And Is Not. Useful Words.

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It can be interesting to put it on your phone or tablet to read when you have some time to spare. Since the file is. Other applications that are reviewed include affinity-based chiral separations and the use of affinity chromatography for the study of drug or hormone interactions with binding proteins.

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Editors: Gupta, Munishwar N. (Ed.) Free Preview. Affinity is the second novel by English writer, Sarah Waters. In this novel, Margaret Prior begins visiting the female prisoners at Millbank prison in an attempt to help her overcome the depression that led to a suicide attempt the year before.

As Miss Prior makes her visits, she becomes aware of a. Authored by a team of respected scientists and technologists, this book covers many pharmaceutical and biotechnology separations methods currently in use. Practical applications and descriptions are offered for air elutriation, microporous filtration, ultrafiltration, phase partitioning, crystalliza.

Publication date Series NATO ASI series. Series E, Applied sciences ; vol. Note "Proceedings of the NATO Advanced Study Institute on Membrane Processes in Separation and Purification, Curia, Portugal, March April 2, "--T.p. verso. Affinity separations utilising immobilised monoclonal antibodies—a new tool for the biochemical engineer Howard Allaker Chase Chemical Engineering Science 39 (), Cited by: Methods for Affinity-Based Separations of Enzymes and Proteins Munishwar N.

Gupta, Ipsita Roy (auth.), described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors.

Separation and Purification Technologies in Biorefineries is an essential resource for scientists and engineers, as well as researchers and academics working in the broader conventional and emerging bio-based products industry, including biomaterials, biochemicals, biofuels and by: Assays employing aptamers in capillary electrophoresis (CE), including competitive and noncompetitive assays, fluorescence polarization (FP) assays, nonequilibrium capillary electrophoresis of equilibrium mixtures, and affinity-polymerase chain reaction-CE assays, are summarized.

These assays can be used to estimate dissociation rate and equilibrium binding constants, determine binding Author: Jeffrey W. Guthrie, Yuanhua Shao, X.

Chris Le. Affinity chromatography which is known as a liquid chromatographic technique for separation and analysis of biomolecules based on their biological functions or individual structures has become increasingly important and useful separation method in pharmaceutical science, biochemistry, biotechnology and environmental science in recent years [].This technique is especially known as the Cited by: 1.

Handbook of Affinity Chromatography by David S. Hage,available at Book Depository with free delivery worldwide.4/5(1). Immobilized metal ion/Metal Chelate affinity chromatography is a separation technique that is based on coordinate covalent binding between proteins and metal ions.

Proteins have a wide variety of amino acids composition which, in effect, generates a range of different affinities towards metal ions. Components of affinity medium When affinity chromatography is used for the purification and separation of large biomolecules from complex mixtures, the support (matrix), spacer arms, and lig and must be considered.

Affinity supports (matrix) Traditionally, affinity chromatography suppor t materials have consisted of porous supportCited by: divided into ion exchange, separations based on size, and even extended to gel-based electrophoretic techniques.

This book will provide a basic introduction to different types of liquid and gas chromatography. The relationship between each type of chromatography is illustrated in .A series of flat-sheet asymmetric mixed matrix membranes (MMMs) have been fabricated with MOF-5, Cu3(BTC)2, and MIL(Al) as fillers and PI polymer as a matrix through the dry–wet phase inversion method.

After the surface modification by coating a PAA solution (15% wt) on the top of the obtained membrane, a thin defect-free selective skin in the MMM is by:

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